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KMID : 0359319980380010209
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Korean Journal of Veterinary Research
1998 Volume.38 No. 1 p.209 ~ p.216
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Induction of twinning in Korean native cattle by transfer of nuclear transplanted embryos
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Abstract
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The purpose of present study is to improve the efficiency of fusion and the developmental rates of nuclear transplanted embryos to produce genetically identical twins from Korean native cattle.
The diameter of aspirated follicles had no significant effect on the recovery rates of oocytes collected by ovum pick-up technique. The fusion rates of nuclear transplanted embryos were significantly higher in 50 and 100㎲ DC duration groups(73.3 and 72.0% ; respectively) than that in 30㎲ group(55.6% ; p$lt;0.05). The cleavage rates of nuclear transplanted embryos appeared to be significantly higher in donor nuclei derived from in vivo (65.0%) than in those from in vitro (50.5% ; p$lt;0.01), but the developmental rates to morulae and blastocysts were not significantly different between them(13.7 vs 10.9%, respectively).
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KEYWORD
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